Science of Field-Flow Fractionation
Journal Cover Pages
Enhance the visibility of your research by transforming your results into great images featured on published journal covers. We invite you to send us your cover images, so we can showcase them here for everyone to see.
If you would like your journal cover to be shown here as well, please contact us at info@fffseparation.net
Book Recommendations
Have you read or written a new book about FFF? This is the ideal place to present it. Contact us via info@fffseparation.net
Featured Papers
“This month’s featured paper highlights AF4 as a powerful tool for characterizing complex therapeutics. The authors discuss the need to integrate AF4 into drug development workflows for comprehensive physicochemical characterization, as it offers a more accurate native-state representation than traditional chromatographic methods. Despite its advantages, AF4 remains underutilized in the pharmaceutical industry due to regulatory inertia, lack of standardized protocols, and complex method development. Its role in quality control and final product validation is constrained, as regulatory agencies do not explicitly require its data. The authors emphasize that standardization, regulatory recognition, and industry-wide validation are key for AF4 to reach its full potential as a mainstream tool in quality control, formulation optimization, and mechanistic studies of next-generation therapeutics.” 
“Everybody is aware of the increasing importance of lipid nanoparticles (LNPs) in medical applications. This study uses Asymmetric Flow Field Flow Fractionation (AF4) to compare two types of LNPs and their interactions with a model protein, bovine serum albumin (BSA). LNP size, morphology, state of aggregation and PDI on BSA corona formation are examined with a subsequent analysis of the shape factors. This study presents another great use of the frit-inlet set-up.” Check it out here: https://doi.org/10.1016/j.chroma.2025.465663
“Elution patterns, Brownian motion, membrane fouling… Things, every AF4 user has to deal with, especially when working with novel analytes. In this paper from Geneva (Switzerland), Gasco et al. demonstrate the suitability of AF4-multiple detection to probe-out eco-corona formation and characterize polydisperse systems containing silver nanoparticle (nAg) hetero-aggregates formed with diatoms derived extracellular polymeric substances (EPS).” Check it out here: https://doi.org/10.1016/j.chroma.2024.465507
“Revolutionizing Wine Analysis with AF4-UHPLC: For the first time, AF4-UHPLC unveils the macromolecular secrets of white wines, combining size-based separation with high-resolution hydrophobicity analysis. This groundbreaking two-dimensional approach provides unparalleled insights into proteins and mannoproteins, preserving their native state. A must-read for analytical scientists and wine researchers seeking innovative tools for complex matrix characterization!” Check it out here: https://doi.org/10.1016/j.chroma.2024.465456
“In this collaborative paper from France, Saydé et al. take on the challenge of reproducibility in 3D cancer models. The team used SdFFF to sort and extract low amounts of homogeneous cancer stem cell subpopulations from heterogeneous glioblastoma cell lines and managed to grow reproducible 3D tumor spheroids in low molecular weight gelators. Check out the full paper here.”
“Do you like red wine and want to know more about the composition of different populations existent in wine? Read the latest research of Valentina Marrassi from Italy published in Food Research International. They applied Frit-Inlet AF4 in combination with MALS and UV detection in order to investigate different colloidal species. Check out the full paper here.”Jobs and Career Opportunities
“FFF channel is looking for a suitable pump to achieve optimum separation results”.
Have you finished your education, are you looking for a new challenge or do you need an FFF expert? The FFF job platform is the right place to go for FFF beginners or experienced professionals. This page is open for companies, universities and research institutes as a job or intern seeking platform. Job seekers can also present their profile anonymously here. For new posts, please contact: info@fffseparation.net
Som stipendiat vil du jobbe med nyskapende forskning på utvikling av lipid nanopartikler og vesikler for målrettet antimikrobiell behandling.
Vi ønsker oss en engasjert kandidat med solid faglig bakgrunn og en lidenskap for forskning. Vi samarbeider med forskningsmiljøer både nasjonalt og internasjonalt, så gode samarbeidsevner er et must! 🌍🤝
📅 Søknadsfrist: 28. februar
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Ta kontakt hvis du har noen spørsmål til stillingen eller prosjektet.
As part of the Horizon Europe Marie Skłodowska-Curie Actions MSCA project “Complex lipid membranes for science and technology” – CLIMB, coordinated by Yuri Gerelli, we are now looking for a Doctoral Candidate with a background in separation science (ideally FFF, AF4, CF3) and light scattering techniques (MALS, DLS, SAXS) for the characterization of novel nanomedicine formulations such as mRNALNP!
You will conduct your research at Postnova Analytics Headquarters in Landsberg and be enrolled at the Johannes Gutenberg-Universität Mainz under the academic supervision of Peter Langguth.
We’re looking forward to receiving your application! More information can be found here!
Mechanical Engineer & Microfluidics Specialist
Dr. Shad is a highly skilled mechanical engineer with a PhD from the University of Utah. Specializing in microfluidic device design and biomedical device optimization, Dr. Shad has developed advanced computational models using ABAQUS, COMSOL, SolidWorks, and ANSYS Fluent, and is proficient in R, Python, and MATLAB.
As a Graduate Assistant at the University of Utah, Dr. Shad led research on ion separation, nanoparticle separation, and the efficacy of local anesthetics, employing high-performance computing and advanced statistical methods. Notable projects include the optimization of CyElFFF devices for ion separation and the fabrication of novel electrode materials to enhance separation performance.
Dr. Shad is also dedicated to education and mentorship, overseeing senior capstone projects and teaching courses in Microfluidics, Fluid Mechanics, Material Science, and Heat Transfer. His work has been presented at international conferences and published in leading journals.
Dr. Shad is open for job opportunities in mechanical engineering and biomedical research
LinkedIn: https://www.linkedin.com/in/mhmsh/